Dear fellow researchers,

during the course of my studies I am planing to conduct histological examinations of tadpoles (a) several stages and (b) several different species. In the short term I want to use standard HE and PAS staining methods and only later in my studies am looking for advanced proceudures like IHC staining etc.

While I know how I am going to fix my tadpoles (as a whole and not disected), I am not sure whether or not the process of decalcification of fixed anuran larvae (with 10% NBF) is necessary, advisable or can be omitted?

I am aware of tadpoles having a mostyl cartilage cranium and general skeletal structure. Which is why I assume decalcification could be omitted. However, with advancing developmental stages of tadpoles up until metamorphosis, the skeletal structures ossify gradually.

I am very greatful of any hints, tips or shared experience in this regards (although any advice for chemical fixation of tadpole tissue is also very welcome).

Thanks in advance and stay healthy!

Best,

Konrad