Been doing assay for my research in using fungal culture as biofumigant. I did a two-bottom plate assay where a seven-day old fungal biocon were covered with a freshly transferred fungal pathogen grown in another plate. I sealed the two bottom plates using plastic wrap. It was so effective that the pathogen didn't grow. Can I attribute the effect to the supposed volatiles produced by my biocon agent? Or the decreased oxygen concentration due to biocon respiration could have also influenced its failure to grow?
comparing with your control plates, make your observations on shorter intervals.
Another solution is to use a control treatment with another fungal strain that doesn't produce anti-fungal volatiles. You may test several isolates to find one with no effect and use it as your control to eliminate the error you are expecting about oxygen utilization.
Good luck
Hello Jo Den, you are welcome.
Most likely you will end up finding that the growth suppression you've observed is related to production of antifungal volatiles
All the best :)
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