I am doing a DNA extraction from potato leaves infected with Phytophthora infestans for quantification via qPCR. The protocol includes two buffers: first, the tissue is disgragated with an extraction buffer (200 mM Tris–HCl, pH 8, 100 mM NaCl, 25 mM Na2 EDTA, 3% p ⁄v SDS and 125 ug/mL proteinase K) and after 1 hour incubation (37°), it is added one volumen of lysis buffer (100 mM Tris–HCl, pH 8, 2,5 M NaCl, 20 mM Na2EDTA, 2% p ⁄v CTAB y 2% v ⁄v b-mercaptoethanol). In this step, The buffer sisn´t mixed but it formed two phases. I noticed that the CTAB buffer is reeeeally dense. Also, it was hard to dissolved CTAB powder for the buffer preparation. I already cheacked that the concentrations pf each reagent was ok and pH of final buffer and solutions that were prepared before was about 8.3.

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