Hello.
I have found studies where N2 was or wasn't used for bovine embryos culture. Can anyone advise me the rationale of using 90% of N2 along with 5% of CO2 and 5% of O2? From the literature it seems that environment enriched with N2 produces more blastocysts, but this is not obvious from all the papers I have studied.
Thank you all.
Jiri
Check this link
http://humrep.oxfordjournals.org/content/15/2/395.full.pdf
This tri-gas system is not necessary, although many labs have a preference in using it. Our experience with N2 is that there are no tangible differences when compared to the system with CO2 and O2. Here we use without the N2 and the production rates of blastocysts always average 35-40% (normal for cattle). I hope it was helpful.
Dear Valter.
Well, you didn't answer my question at all. Thank you anyway for sharing your experience with your blastocyst rate.
Regards,
Jiri
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