DNA/RNA G4 modelling and visualization tool ?

Hi, I would like to ask if anyone knows any tool for visualizing/modelling guanine structures (any RNA structures with Hoogsteen base pairing) based on sequences? I mean, if there is any...

02 November 2020 1,136 2 View

Is the length of first DCA axis always bigger than the second?

I'm performing a DCA analysis of a data-matrix of 150 releves and the result is 3.3 SD units for the first axis and 3.8 SD units - for the second. The configuration of releves is reasonable and...

28 October 2019 4,809 1 View

RNA isolation from blod: How to precipiatate excess protein before loading samples on silica column?

05 October 2017 3,989 2 View

Does anyone have any experience with rRNA depletion prior to transcriptome NGS?

16 October 2016 5,033 3 View

Is there any chance to melt Ni3Al dice (4,5 mm) on oxide ceramics in resistance furnace under Ar atmosphere ?

We used a lot of types of oxide ceramics based on Al2O3 and even used Spinel (MgAl2O4) ceramics - refractory concrete, which is even more stable, but Ni3Al always oxidized and not melt. We are...

09 February 2016 6,274 3 View

Is anyone interested in collaboration in the area inquiry-based learning?

I am interested to scientifically collaborate (projects, comparative research, training) on the topic of "inquiry-based learning" - IBSE. Anyone interested?

15 June 2015 5,340 3 View

Are there specific photographic techniques of surveys on for public perception of forest?

I prepare questionare surveys about public perception of forest within project http://rafa.frrms.mendelu.cz/en?lang=en. I have ideas about objectives of questionare, but Im looking for rules...

26 March 2015 2,524 3 View

Has anyone used psychoanalytic methodology for social science research?

I'm exploring methodological issues and looking for sources and literature related to psychoanalytic methods used in social science research. Thank you in advance for any ideas you might have!

03 December 2014 9,742 14 View

What is the least drastic method to kill decapod crustaceans for subsequent preservation?

A variety of methods are used to kill decapod crustaceans for subsequent preservation. Generally the most acceptable method of killing crustaceans is to chill the animals to the insensitivity and...

24 October 2014 2,021 20 View

Problems with mRNA determination after precipitation with vivid violet.

Does anyone use vivid violet (EURx) for mRNA precipitation? What is the difference between absorbance at 260nm of pure mRNA and with vivid violet as co-precipitant? Does it disturb the absorbance...

03 April 2013 9,175 0 View

How to classify and recognize unseen image class in document sorting?

To sort the scanned document image I have to determine the class of document. But no of classes of image will keep on adding even after training. So at time of inference first I will detect unseen...

16 February 2021 4,501 3 View

Is CXCR5 on T cells affected after a cell sorting?

I have tried to sort CD8+ CXCR5+ in the ARIA but the purity of my sample is about 50%. After sorting, I stain my samples to verify purity but it is low. So, I thought about "downregulation" or...

15 February 2021 8,034 7 View

Which database could provide the signal sequence for targeting various cellular regions? ?

I am searching for thee database which could provide me signal sequence specific to the various cell organelle. such as signal sequence for mitochondria, signal sequence for endoplasmic...

04 February 2021 6,650 2 View

Are there well-established collaborative sorting tasks/datasets?

Hi, I am currently working on research on communication behavior in Mixed Reality. I am planning a study, where dyads perform a task, in which they have to collaboratively cluster or sort cards...

31 January 2021 4,979 3 View

Is there a technique to store fixed cells after flow for later RNA analysis?

I am separating neuron populations from mouse brains using FACS. I am trying to find a fixation technique for intracellular antigen staining and FACS that will allow long term storage afterwards...

21 January 2021 9,722 3 View

Are Signal Peptide and Transit Peptide different? And what are their functions?

I want to learn about mutation in transit peptide. Can mutation in transit peptide affect the function of the protein?

20 January 2021 396 3 View

Fluorophore panel selection?

Hello, I'm trying to select fluorophores for a large panel of flow cytometry, so I'm wondering what is the maximum degree of overlap between two fluorophores that is considered acceptable? Thanks.

19 January 2021 3,475 3 View

How do you improve retroviral expression from PG13 cells?

I have been trying to express a gene (GFP) in a retroviral system by transfecting human Phoenix-ECO cells, and transducing murine PG13 cells with the supernatant. I then sort GFP-positive PG13...

18 January 2021 476 1 View

Can anyone help me to remove a hydrophobic peptide from C18 column?

I have injected a hydrophobic peptide into analytical C18 column and i have tried to cleaning the column applying a lot of protocols using acetonitrile, methanol, isopropanol, but in the blank...

05 January 2021 3,305 3 View

Metabolomics Data Normalisation?

Hello, Setting: We have a rather large dataset consisting of somewhat 500 samples containing metabolomics data. The data was obtained by analysing dry capillary blood using HPLC methods, yielding...

04 January 2021 8,773 5 View