Is it necessary to prepare FMO controls for each sample to be analyzed when there are two or more samples available?

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What could be the reason for getting low sample absorbance values than blank or zero standard in pre-coated sandwich ELISA kits?

Hi, Dear all, I want to measure human serum IL-1beta and TNF-alpha by using precoated sandwich ELISA kits from BD. I am getting serum absorbance values lower than the zero standard/blanks. I am...

08 September 2015 6,984 3 View

Is it possible to use Hoechst 33258 as live/ dead marker with BV421, 605 and BUV737 labeled antibodies for flow cytometry?

Hi, We are using BV421, BV605 and BUV737 labelled antihuman antibodies from BD for immunophenotyping of peripheral blood. Can we use Hoechst 33258 as live/dead stain with these flourophores? If...

07 August 2015 7,617 4 View

Can anybody suggest M1/M2 monocyte - macrophage surface markers for flow cytometry?

Hi, I want to investigate the monocytes/macrophages in the peripheral blood. The usual markers used are CD14 and CD16 but they are more specific for monocytes. If we want to observe macrophages in...

11 December 2014 4,455 9 View

Hi can any body tell me how to optimize the dose of alloxan to develop stable diabetes in albino rats?

Alloxan induced diabetic animal model is used to screen out drugs for antidiabetic activity. I am facing problem regarding development of stable diabetes in the albino rats upon alloxan injection....

08 September 2011 7,091 15 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View