Shafqat Rasul

5 Questions 11 Answers 0 Followers

Questions related from Shafqat Rasul

Is it necessary to prepare FMO controls for each sample to be analyzed when there are two or more samples available?

Hi, I am analysing human peripheral blood by flow cytometry and I have to prepare FMO controls. If I have two or more samples from different persons then should I need to prepare FMO controls for...

10 October 2015 1,934 2 View

What could be the reason for getting low sample absorbance values than blank or zero standard in pre-coated sandwich ELISA kits?

Hi, Dear all, I want to measure human serum IL-1beta and TNF-alpha by using precoated sandwich ELISA kits from BD. I am getting serum absorbance values lower than the zero standard/blanks. I am...

09 September 2015 7,032 3 View

Is it possible to use Hoechst 33258 as live/ dead marker with BV421, 605 and BUV737 labeled antibodies for flow cytometry?

Hi, We are using BV421, BV605 and BUV737 labelled antihuman antibodies from BD for immunophenotyping of peripheral blood. Can we use Hoechst 33258 as live/dead stain with these flourophores? If...

08 August 2015 7,668 4 View

Can anybody suggest M1/M2 monocyte - macrophage surface markers for flow cytometry?

Hi, I want to investigate the monocytes/macrophages in the peripheral blood. The usual markers used are CD14 and CD16 but they are more specific for monocytes. If we want to observe macrophages in...

12 December 2014 4,491 9 View

Hi can any body tell me how to optimize the dose of alloxan to develop stable diabetes in albino rats?

Alloxan induced diabetic animal model is used to screen out drugs for antidiabetic activity. I am facing problem regarding development of stable diabetes in the albino rats upon alloxan injection....

09 September 2011 7,125 15 View