During construction of fowlpox vector carrying foreign genes under synthetic early late vaccinia virus promoter, is it necessary to include a spacer region of
Hey there! Checkout my latest Creation Get Started to - Learn Data Science with Vidya ! (Hands-on Practical...
21 December 2023 4,885 0 View
Looking for some suggestions, for a colleague! She is working on Minicircle DNA technology. The parental plasmid has the size of 5237 bp and the Minicircle is of size 3495 bp. She is using...
21 July 2023 8,421 2 View
I have been looking for the preparation of extracts from roots, leaves, barks. So pl suggest me the best solvent to extract the samples.
27 January 2023 4,500 1 View
As I'm new to this, i need some guidance about journals.
23 June 2021 6,659 7 View
Hi All, I recieved C elegans from CGC, US. They were very active after I added few drops of cultured OP50 bacteria. On observing under the microscope, I could see lots of active small and big C...
28 March 2021 2,111 0 View
I have been working on creating on knocking out the ThyA gene (an essential gene) in e. coli using lambda red but have only been successful with lab type strains (MG1655 and BW25113), but...
08 January 2020 2,704 4 View
I am looking for few reputed journals to publish my work
13 December 2018 5,750 3 View
Dear Sir .. I too research in this same area...but include with security features to EHR ... i need ur help toimplement my work... how to work with EHR?
31 January 2018 9,964 3 View
I designed the inverter and I am trying to design parallel these standalone inverters, I have read many papers and have an idea of actually how it works and controlled, but somehow I have trouble...
10 January 2018 6,582 3 View
Thanks in advance.
20 November 2017 4,998 5 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...
04 August 2024 8,997 1 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...
14 July 2024 8,482 3 View
I'd like to know if anyone has got experience in intracerebroventricular (ICV) AAV inj. I am looking for a concomitant spread of the AAV including but not limited to the motor cortex and the...
12 July 2024 781 0 View
I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...
11 July 2024 5,669 5 View
Hey everyone, I've been working with GRIN lenses (Inscopix) for almost 1 year and still had no luck seeing strong fluorescence on them. When I look at them, I can see good blood flow and,...
08 July 2024 5,178 2 View
I get two different bands from BCMV after PCR
07 July 2024 2,112 1 View
We have a couple of primers but when we make a blast for those primers we see two results: When I use Blast from NCBI, I found many lineages which anniling with many lineages. But when I want to...
01 July 2024 7,644 3 View
Factor loading is more than 0.92 also ave and cr are more than 0.95? Is this acceptable? If it's not i tried to delete items but the more i delete the more factor loading is higher! how can i...
20 June 2024 3,345 4 View