6 Questions 5 Answers 0 Followers
Questions related from Ranjani Rajasekaran
Looking for some suggestions, for a colleague! She is working on Minicircle DNA technology. The parental plasmid has the size of 5237 bp and the Minicircle is of size 3495 bp. She is using...
22 July 2023 8,394 2 View
During construction of fowlpox vector carrying foreign genes under synthetic early late vaccinia virus promoter, is it necessary to include a spacer region of
04 July 2018 360 0 View
I want to insert 4 restriction sites between my two genes. Initially, I thought of inserting MCS into my vector and then cloning my two genes on either side of the MCS. But, is it possible to add...
26 May 2017 8,510 5 View
I want to design a polylinker sequence to be inserted into a vector.Should I provide overhang prior to each restriction enzyme, as I have done in this?Or overhangs at 5' and 3' is enough?5'...
05 April 2017 9,084 3 View
I inoculated NDV containing AAF into 9 day old SPF egg. After 72 hours of incubation, the AAF was harvested. Amplification of M and FPCS gene showed a positive PCR product. But, I am not able...
02 September 2016 8,553 2 View
Can someone suggest a recent reference for construction of a fowlpox vector with multiple antigens?
10 June 2016 4,334 1 View
