Ranjani Rajasekaran

8 Questions 5 Answers 0 Followers

Questions related from Ranjani Rajasekaran

Will Macroconidia of Dermatophytes be visible on a tape impression smear taken from a dog?

A dog was brought with a history of dandruff and itching. Tape impression smear was taken from the dorsum region. Upon staining it with Lactophenol Cotton Blue stain, I found Macroconidia. But I...

04 February 2025 3,284 0 View

How to avoid genomic DNA contamination in a Midiprep plasmid extraction?

Looking for some suggestions, for a colleague! She is working on Minicircle DNA technology. The parental plasmid has the size of 5237 bp and the Minicircle is of size 3495 bp. She is using...

22 July 2023 8,451 2 View

Is it necessary to include a terminator signal sequence along with spacer to terminate foreign gene transcription in fowlpox vector construction?

During construction of fowlpox vector carrying foreign genes under synthetic early late vaccinia virus promoter, is it necessary to include a spacer region of

04 July 2018 408 0 View

Is a modification at the 3' end of F7 ORF of fowlpox virus gene necessary to prevent its overlap with the next gene?

Between F7 and F9 gene of FWPV, there is a 32bp non-coding region. It is said that in this region foreign genes could be inserted. Immediately downstream of the stop codon of F7 ORF, foreign genes...

04 July 2018 9,392 0 View

Can I add more than two restriction sites in my primer?

I want to insert 4 restriction sites between my two genes. Initially, I thought of inserting MCS into my vector and then cloning my two genes on either side of the MCS. But, is it possible to add...

26 May 2017 8,556 5 View

Is it necessary to provide overhang between each restriction enzyme in a polylinker sequence?

I want to design a polylinker sequence to be inserted into a vector.Should I provide overhang prior to each restriction enzyme, as I have done in this?Or overhangs at 5' and 3' is enough?5'...

05 April 2017 9,126 3 View

How to identify the replication of NDV in embryonated eggs?

I inoculated NDV containing AAF into 9 day old SPF egg. After 72 hours of incubation, the AAF was harvested. Amplification of M and FPCS gene showed a positive PCR product. But, I am not able...

02 September 2016 8,613 2 View

Can someone suggest a recent reference for construction of a fowlpox vector with multiple antigens?

10 June 2016 4,387 1 View