Name few sci journals where plastic waste recycling, plastic waste blends, compatibilizer oriented works will be more relevant?

I am looking for few reputed journals to publish my work

13 December 2018 5,715 3 View

Can I add more than two restriction sites in my primer?

25 May 2017 8,499 5 View

How to identify the replication of NDV in embryonated eggs?

I inoculated NDV containing AAF into 9 day old SPF egg. After 72 hours of incubation, the AAF was harvested. Amplification of M and FPCS gene showed a positive PCR product. But, I am not able...

01 September 2016 8,541 2 View

What happens to the DNA from sloughed off intestine epithelial cells when extracting bacterial DNA from mouse fecal samples?

I am currently using the PowerSoil DNA extraction kit from MoBio for DNA extraction from bacteria present in mouse fecal matter. I wanted to know how the sloughed off intestine epithelial cells...

21 August 2016 5,137 1 View

How can I specify the orientation and polarization of the doubly clamped piezoelectric structure?

Hi all, I work on piezoelectric energy harvester which has a doubly clamped beam structure with proof mass at the center. I simulate using both COMSOL and ANSYS but facing few issues. I use AlN as...

07 August 2014 7,112 4 View

mRNA to protein expression?

I have been conducting a series of experiments where I transiently transfect plasmid with my gene of interest into HT29 cells. When I performed qRT-PCR on these cell samples, I can clearly see an...

03 December 2013 6,975 8 View

Can protein expression stop in cell lysates?

I collected whole cell lysates of HT29 Clones in RIPA buffer (Protease Inhibitor cocktail, PMSF, Na3VO4 and NaF all added into the RIPA buffer) and analyzed them by western blot on the same day...

19 November 2013 8,644 4 View

Can anyone suggest the reason for E9 cell death after 32 hours transfection?

I am transfecting E9 cells with K44a with pcDNA 3.1 backbone. The transfection reagent is PEI, the purity of the plasmid is 1.9 and conc is 340 ng/ul. I used 0.5 and 1 ug conc of Plasmid and 1.5...

10 October 2012 2,008 5 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

What is recommended for solid domain in ANSYS FLUENT?

Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?

01 March 2021 9,785 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View