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Questions related to Liquids
average particle size calculation from TEM
05 August 2024 2,823 1 View
What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?
02 August 2024 1,630 1 View
or we can collaborate
02 August 2024 6,612 2 View
After deprotection of the tert-butyl ester to generate the carboxylic acid in chloroform with TFA, my compound‘s pyridine moiety forms a TFacetate ammonium salt (small molecule drug, not a...
30 July 2024 9,662 3 View
I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...
29 July 2024 5,568 0 View
I'm working on the atmospheric water harvesting, and i synthesized MOF by adding some materials into precursor and changing the conditions. (i followed Hydrothermal method) Actually, if MOF is...
25 July 2024 1,423 0 View
So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...
19 July 2024 3,983 3 View
As, my studies are related to water adsorption, the substrates should be hydrophilic in nature. Ni-foam being hydrophobic in nature, the results are not as expected as in literature. Hence i...
15 July 2024 8,756 4 View
DNA isolation requires many freshly made buffers. So is it necessary to autoclave each vessel used, before preparing buffers in them?
11 July 2024 7,434 3 View
Salam everyone, So I am trying to extract DNA from isolates of clinical samples of staphylococcus aureus, I am using a QIAamp UCP kit. I followed the same manufacturer protocol and used Qubit to...
04 July 2024 3,801 1 View
I have done site-directed mutagenesis (Substitution) to obtain a Cysteine mutation in my gene of interest. Now, I have two domains in my protein, And I want to delete one of the domains to obtain...
06 June 2024 9,340 1 View
Hey there, I am currently conducting a life cycle assessment and as advised, I used different life cycle impact assessment (LCIA) methods to check for robustness of results. I found that when...
04 June 2024 8,336 2 View
How does zero tillage help in carbon sequestration and significance of conservation tillage in reducing carbon emissions?
03 June 2024 735 4 View
Can I store the freshly collected tissue sample in trizol instead of RNA later buffer? if Yes, How long can i store? will it affect on quality/quantity of the RNA sample? Which is better among...
03 June 2024 491 3 View
Cloud Computing
31 May 2024 310 7 View
Dear fellow researchers, We are currently operating a Philips XL30 Scanning Electron Microscope (SEM) equipped with a tungsten filament and have encountered a significant issue. Recently, our...
29 May 2024 9,321 2 View
I perform al alkylation reaction on an imdazole compound where the alkyl reagent (1-Iodo-Octane) was in excess. After the reaction was completed, I then performed an extraction, followed by...
25 May 2024 7,950 2 View
Hello every, I am looking for a commercial enzyme that can degrade sulfate dextran. What is known at present is that dextran can be a substrate for dextranase. so I want to know if sulfate...
24 May 2024 1,383 0 View
The size miRNA is 89 bps and the primers I have designed (using traditional method for designing gene primers) should give an amplified product of 71 bps. But I am getting product of higher...
24 May 2024 7,477 3 View
In order to proceed with the quantification of an Azide Alkyne Cycloaddition reaction efficiency by using a fluorogenic probe with Cy5-DBCO, I first need to construct a calibration curve. The...
17 May 2024 6,166 5 View
En mi opinión son respeto y responsabilidad ambiental y usted que opina. Le adjunto dos publicaciones de mi autoría, para que me pueda dar su criterio al respecto.
08 May 2024 5,911 1 View
Recently I am studying on Exchange interaction and Magnetic force theorem(MFT) .. there the basic idea of MFT is we have to rotate the exchange-correlational (xc) potential by assuming that within...
30 April 2024 8,479 0 View
People keep suggestion we switch from the Reconyx Pro cameras we use to monitor mammal populations to Spypoint cameras to enable solar charging and cellular distribution of photos. We are not...
24 April 2024 2,096 0 View
I need numbers relating to works already completed, possibly hotels or large structures.
22 April 2024 2,583 1 View