How do I dissolve cannabidiol (CBD) and crude hemp extracts in 1:1:18 ethanol:tween80:0.9% saline for IP injection in mice?

I have tried dissolving CBD and crude hemp extracts in 1:1:18 ethanol: tween80: 0.9% saline. I dissolved the drugs in ethanol first then tween, after 10 mins sonication the solutions looks ok but...

27 January 2021 6,259 3 View

Where to find data set for big data analytics ?

I am looking for a data set (preferred to be spatial data) in order to apply big data analytics on it, can you help me to find such a data set please?

05 January 2021 4,860 4 View

How do I properly normalize spectra/peaks for comparison (Photoluminescence and Electroluminescence) ?

Hello, This questions pertains to comparing peaks from spectra of 2 or more different samples in a fair way through normalizing I know Origin normalizes based on the highest peak however if I...

12 December 2020 403 1 View

Can xylose be formed from fructose over acid catalysts?

I am new in the field of sugar chemistry. I wanted to know if it is possible for fructose to be converted to xylose over acidic conditions. I saw in some schemes a pathway from fructose to xylose...

07 December 2020 5,247 3 View

Why I obtain very high surface area under HMF peak in HPLC even with suitable dilution?

I am converting Fructose into HMF, Levulinic acid and formic acid are possible side products. I analyze the data via HPLC, bio rad column, I got nice results for some reaction set. But when I...

23 September 2020 6,375 7 View

What is so special about graphene?

Why is graphene so important?

18 September 2020 3,857 9 View

What is the difference between ultra-high vacuum chamber and edwards vacuum coating unit if we are using the the technique of thermal evaporation ?

developing thin films in ultra high vacuum chamber thermal evaporator.

09 September 2020 4,172 3 View

Can anyone help me out to find out my masters thesis research topic??

My area is software engineering, I haven't any idea about the thesis. need lots of help. I want that the topic should be Lil bit easy. :P Thank You

06 September 2020 4,678 14 View

What are the best topics for research on environmental informatics, immuno informatics and nano informatics now a days alongwith the best methodology?

best research topics and methodologies on pollulatnts which influence genetic diseases and the treatment through nano technology?

09 August 2020 5,326 1 View

How to extract features from an image and save these features as a file, do you have any python code?

My question is: I need to extract features from an image and save the features of each image in a separate file so that later on, I can apply arithmetic operations on these features. I need a...

05 July 2020 3,303 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

QPCR reaction efficiency with odd results what does this indicate?

I am working on a CFX 96 machine to determine reaction efficiency of my gene of interest. After performing a serial dilution of my cDNA template, I was able to find great reaction efficiencies for...

23 February 2021 809 3 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View