I need to express a gene which code for a inner membrane protein in a transgenic bacteria and I also do not know the promoter of the bacteria. I am going to use a expression vector with a promoter. If anybody know please explain me;
Do I need a help of a signal peptide to target the protein to the inner membrane. If yes what are the signal peptides I can use?
Which type of vectors will be suitable?
Thank you.
Thanks Mangal,I checked my sequence in signal P but no peak for signal peptide was resulted. And I will go through all your other suggestionside too.
My argument is; are each inner integral membrane proteins contain a signalpeptide. Signal peptide is use for localization. If the folded protein cannot exist in the cytoplasm it can directly integrate into inner membrane of bacterial cell. So then is it need any signal peptide?
Mangal is correct for most membrane proteins (MP) transmembrane segments (TMDs) serve a purpose of signal peptide (SP). These TMDs satisfy both targeting and anchoring function. There some cases (type I topology) where SP is cleaved by signal peptidase. To my knowledge at least for one polytopic one inner membrane protein in E.coli the presence of cleavable SP was proposed but not addressed experimentally.
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