Yes you can do that to inhibit the growth of other microorganisms in sample
You'll be hard pressed to find a fungal isolation protocol that uses selective agent only in sample. In isolation - if you use a selective gent, you're objective is to stop growth of unwanted contaminants in the isolation medium where efficacy had been established, not the original sample where its addition has not been shown to have validity.
https://www.afwgonline.com/resources/articles/fungal-isolation-protocol/
https://pdfs.semanticscholar.org/5b65/0cda764d01946f42e636f284e676ebbf3c52.pdf
http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.675.2051&rep=rep1&type=pdf
Hallo - use Saburaud agar with chloramfenicol + gentamycin or for dermatozes Saburaud agar with actidion (nr 2 inhibits growth of moulds).
Wojtek
Hi Philemon Orwa. Please read this writ up carefully.
I read above discussion. It was nice. It'is better to add antibiotics in Media or in sample. It's not a big question. As are incubating any media for incubation. Incubation is necessary growth of microorganisms. Right.....!
Every antibiotic retain its potential till few days depends on type and concentration of antibiotics.
If we add antibiotics (may be with combination) in sample. We are spreading only 100 micro litres of sample on media. There are full chances of contamination. Because it sterilise only sample not media. After inoculation there are may encounter occurs and cause contamination in petri plate. As your media is not having antibiotics and it get contaminated.
On other hand, if you incorporate antibiotic in the media before plating in molten agar (temperature approx. 45-50 degree Celsius). You will not be bothered by contaminants and other encounters with your plate in you incubate plate for a month or more.
Therefore, I suggest you can incorporate antibiotic in media regardless of the microorganism you want to isolate from your sample.
I hope you will be satisfied. If you have any query pleas ask....
you can add specific antibiotics to media to eradicate undesirable microorganisms because they will cause contamination in media ,but you must choose the antibiotics which not affected fungi you want to isolate
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