Does beta mercaptoethanol retain its reducing ability at PH 4.5?

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Hitesh Verma

I dont think beta mercaptoethanol would be effective...

The pKa is i think above 7..

A better alternative is TCEP..which is phosphine derivative..

Although its lot more expensive but its mechanism is such that it can work on a broad pH range...

Good luck..

Rajivgandhi Sundaram

you could add DTT instead of BME or even TCEP also a best choice

Orwa Barakat

Thanks for your answers. Actually, DDT is the worst choice at acidic PH. I know that TCEP is the most PH flexible reducing agent but I want first to make sure that BME does not work at acidic PH. I could not find the redox potential of BME at acidic PH.