I have DNA sequences of ITS1 genes both forward and Reverse sequences. How do i build combined phylogenetic tree from these?

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Ramesh Balasubramanian

Use https://doua.prabi.fr/software/cap3 for assembly. Then BLAST with contig. Download the top 10 sequences and use MEGA 11 for tree building.

Ricardo Franco-Duarte

It depends on your goals. After you obtained the consensus sequence (from forward and reverse), you can perform Blast analysis to find the most closely related sequences from other strains/species. Download the number of sequences you find useful to your analysis (to be included in the tree), align them using for example MEGA as proposed by Ramesh, and build also the tree in MEGA. Write me if you need any additional help or guidance.