What are the factors responsible for non uniform decrease in corrosion rate?

I am working on the corrosive effect of food condiments juices on some selected metals using weight loss experiment. But while plotting the graph, I found out that the decrease in corrosion rate...

21 January 2021 6,688 4 View

Granger causality lags for comparing multiple simulations?

Hi all, I'm running neural network simulations and using granger causality to compare the Granger drive from one population to another. Generally speaking I'm using a code to find the Akaike...

16 November 2020 925 3 View

Creating randomization matrices (null models) with abundance data in R (Ulrich & Gotelli 2010)?

Hello, We are aiming to create a simple randomization algorithm in R program with abundance data. Specifically, we are aiming to create the IT-Algorithm (Ulrich & Gotelli 2010, Ecology),...

25 March 2020 5,873 3 View

Are there special considerations for Filipin stock solution storage and reuse?

My lab recently purchased both the Sigma (F9765) and Cayman (70440) Filipin reagents. These reagents were resuspended in DMSO in the hood in the dark and aliquoted to avoid freeze/thaw. Stock...

05 November 2019 8,392 1 View

Can mouse T cells be expanded from splenocytes in T75 flasks?

I need to activate and expand a large number of transgenic T cells from mouse splenocytes for adoptive transfer. I normally do this by pulsing splenocytes with antigen in 96 well plates. However I...

24 April 2019 4,482 3 View

Quantifying trend over time for bray curtis values?

Hello, I'm analyzing bray-curtis values over time. I would like to quantify if 1) there is a rapid or gradual change in bray curtis values, and 2) how long this change occurs. As a hypothetical...

19 February 2019 7,189 3 View

What causes this variable feature at the start of LiCoO2 charging voltage profiles?

I have made many LiCoO2 vs Li coin cells for charging and studying structual changes during the Li removal, but my voltage profiles do not follow the conventional behaviour in the literature. I...

09 September 2018 7,740 2 View

Setting Up LMM in SPSS: Comparing Two Groups (12 Individuals Each) Across 16 Time Periods?

Hello, My Project: I'm analyzing beetle species at carcasses predated on by GPS collared pumas in the Greater Yellowstone Ecosystem. I have 12 fresh carcasses that I've sampled each week. Each...

02 January 2018 9,524 3 View

After you create a mokken scale, can you get an overall score for the scale?

I'm exploring the concept that scales can be ordered and that certain items should carry more weight in a scale. I came across guttman scalograms and Mokken scaling techniques. Creating the...

22 November 2017 7,026 5 View

What causes false negatives in PCR reactions and how can I fix it?

I've recently been performing many pcr reactions and have only recently run into contradicting results. What once was coming out as positive is now negative. I run positive and negative controls....

05 April 2017 786 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

No title

03 March 2021 5,360 2 View

No title

02 March 2021 3,060 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View