Josh Gammon

9 Questions 4 Answers 0 Followers

Questions related from Josh Gammon

Can homozygous thy1.1 cells be distinguished from thy1.1/thy1.2 heterozygotes by FACS?

I want to breed a transgenic mouse strain that expresses a transgene as well as the thy1.1 congenic marker. I will breed the transgenic mouse with a thy1.1 mouse on the same background, and I...

16 July 2019 7,811 1 View

How long is s6 phosphorylated in T cells after CD3/CD28 stimulation?

I am wondering if I stimulate T cells in vitro with anti CD3/CD28, how long will the s6 ribosomal protein be phosphorylated. I would like to look at ps6 after 4 days or stimulation to correlate...

30 May 2019 7,701 2 View

Can mouse T cells be expanded from splenocytes in T75 flasks?

I need to activate and expand a large number of transgenic T cells from mouse splenocytes for adoptive transfer. I normally do this by pulsing splenocytes with antigen in 96 well plates. However I...

25 April 2019 4,660 3 View

What is the best method for analyzing pancreas infiltrating T cells by FACS?

I would like to study the effect of a treatment on the infiltration and phenotype of T cells in the pancreas in NOD mice using FACS. I am not sure what the best method for processing the tissue...

25 April 2019 369 6 View

Can mouse T cells be stained with CCR4, CCR5 and CCR7 for FACS along with FoxP3 using a transcription factor fixation/permeabilation buffer?

I have stained mouse T cells for CCR7 many times, but have heard mixed things about the compatibility of this antibody (clone 4B12, BD). I am also wondering about CCR4 (clone 2G12 biolegend) and...

03 April 2019 3,622 0 View

Is IL-2 necessary for short term expansion of T cells with anti CD3/CD28?

I would like to investigate the ability of an experimental treatment to inhibit activation and proliferation of naive mouse T cells in vitro. I was wondering if I coated culture wells with 2ug/mL...

12 February 2019 9,181 1 View

Can IL-7 prevent naive T cell death in vitro?

I would like to investigate the ability of an experimental treatment to inhibit T cell proliferation/survival in vitro after stimulation with anti-CD3/CD28. I also want to determine if the...

12 February 2019 5,625 2 View

How stable are phospholipids?

I have a collection of phospholipids (DOPC, DSPE-PEG) and cholestorel that I mix together to synthesize liposomes. Normally I recieve the lipids aliquotted dissolved in chloroform in the correct...

09 January 2019 2,931 3 View

What is the minimum number of antigen specific T cells to detect inflammatory cytokines in co-culture?

I want to do a DC:T cell co-culture and quantify the secretion of IL-17 and IFNg after culture. However the antigen specific cell population I will be using is rare (I expect to be able to recover...

10 December 2018 6,605 3 View