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Questions related from Josh Gammon
I want to breed a transgenic mouse strain that expresses a transgene as well as the thy1.1 congenic marker. I will breed the transgenic mouse with a thy1.1 mouse on the same background, and I...
16 July 2019 7,779 1 View
I am wondering if I stimulate T cells in vitro with anti CD3/CD28, how long will the s6 ribosomal protein be phosphorylated. I would like to look at ps6 after 4 days or stimulation to correlate...
30 May 2019 7,673 2 View
I need to activate and expand a large number of transgenic T cells from mouse splenocytes for adoptive transfer. I normally do this by pulsing splenocytes with antigen in 96 well plates. However I...
25 April 2019 4,582 3 View
I would like to investigate the ability of an experimental treatment to inhibit activation and proliferation of naive mouse T cells in vitro. I was wondering if I coated culture wells with 2ug/mL...
12 February 2019 9,134 1 View
I have a collection of phospholipids (DOPC, DSPE-PEG) and cholestorel that I mix together to synthesize liposomes. Normally I recieve the lipids aliquotted dissolved in chloroform in the correct...
09 January 2019 2,887 3 View
I want to do a DC:T cell co-culture and quantify the secretion of IL-17 and IFNg after culture. However the antigen specific cell population I will be using is rare (I expect to be able to recover...
10 December 2018 6,581 3 View