Is GC content important when designing oligonucleotides for transfection?

More Çiğdem Yılmaz's questions See All

In the cerenkov.mac file of GEANT4 optical examples, can we change the scintillator and PMT material from LXe to NaI?

In the optical examples of GEANT4, specifically in the cerenkov.mac file where the LXe geometry is defined, is it possible to change the geometry and material of the scintillator and PMTs from LXe...

08 May 2024 7,236 0 View

Concentrating exosome for Western-Blotting?

Hello, I have isolated extracellular vesicles from a cell culture using the Exosome isolation kit. However, the western blot results did not show up because the concentration was only 0.2 ug/ul. I...

15 February 2024 8,378 4 View

(SSR marker) I used the same pair of primers and observed different band patterns in PCR. What is the reason for this?

In the SSR method, I used the same pair of primers (forward and reverse) in two different individuals, but I observed different band patterns in PCR.

05 February 2024 3,438 2 View

What is the reason for the changes in the band despite the use of the same SSR primer in both genotypes?

Genetic Engineering. Assuming that there were no errors during the process.

03 February 2024 2,832 0 View

After which processes can mRNAs obtained as a result of gene expression be sequenced?

Genetic Engineering. Sequence. mRNA sequence.

03 February 2024 1,375 2 View

What is the technical difference of CAPS and SCAR markers in genetic engineering?

Genetic Engineering. Markers.

03 February 2024 4,012 0 View

Why are 2 different restriction enzymes used when preparing genome libraries?

Genome library. Restriction Enzymes. Genetic engineering.

03 February 2024 5,429 2 View

Problems on Plant Based Protein Hydrolysis With Enzymes?

Dear All, I want to kindly ask a question about enzymatic hydrolysis of plant based proteins. We have proteases as Flavourzyme and we hydrolyze pea and wheat mixed protein. We construct a process...

01 October 2023 1,029 1 View

How can I detect different tones of the same colour that are very close to each other by image processing?

How can I detect different tones of the same colour that are very close to each other by image processing? I want to get the position of each tone in the flow.

13 August 2023 7,609 0 View

Why does DEA frontier find the efficiency of all DMUs 1?

I calculate the efficiency of public transportation vehicles via the DEA method and I use DEA frontier. It calculates the efficiency of all DMUs as 1. Can anyone identify what the problem is and...

05 June 2023 1,542 3 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

29 July 2024 3,229 1 View