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Questions related from Çiğdem Yılmaz
Hello there. After adding the protease inhibitor cocktail to the ripa solution, I aliquoted it at room temperature and kept at -20 degrees. However, my aliquot process at room temperature took...
24 August 2020 5,595 4 View
I cannot detect Parp protein after total protein isolation Hello everyone; I cannot detect the Parp protein after total protein isolation. I can see my cells die morphologically after a novel...
19 July 2019 2,637 2 View
Protocol content (NEB DNAse I reaction Protocol) : RNA: 10ug DNAse reaction buffer 1X 10ul DNAse (Rnase free) 1ul Nuclease free water 100 ul My question is; if I use this protocol after isolating...
12 January 2019 1,441 3 View
HEK-293T is becoming this for a while after being in culture. (20X magnification) Other cells in the culture have no such problem. Can you describe this morphology?
01 January 1970 2,943 5 View
Is GC content important when designing oligonucleotides for transfection? Is this a problem for oligonucleotide-based transfection, where the dna region I will design oligonucleotide is very rich...
01 January 1970 1,027 3 View
Do you need specially designed vectors for Crispr / Cas9 ? If so, why? Can't I clone the gene into the restriction site of a vector I have?
01 January 1970 6,399 2 View
Hello to everyone! I have an insert that I transformed into E. coli STBL3 strain. These are liquid cultures that I stored at -80 ° C.These are liquid cultures that I store at -80C. I wonder,...
01 January 1970 7,905 15 View
Is it a problem if there is a PAM sequence in the gRNA? Cas9 is a protein that ultimately recognizes PAM sequences in DNA by gRNA and binds to and cleaves DNA. In this case, do I need to eliminate...
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