I have always isolated ASC by performing a collagenase digestion on the tissue, then pipetting up and down 75-100x before centrifuging. After centrifugation adipocytes will float and the ASC cells will be in a nice pellet. I have never performed without a digestion.
Hello Muhammad, I use tissue explant method for isolating adipose stem cells. All I do is just chop the adipose tissue into 1-2 mm2 fragments, and then place them in a 6 well plate containing 1 ml of alpha- MEM complete media. The tissue is then removed from the well once the cells start appearing. Its relatively easy and inexpensive method, but it usually takes around 15- 20 days to obtain confluent culture.