Aditi Mahajan

5 Questions 15 Answers 0 Followers

Questions related from Aditi Mahajan

Can anyone please suggest appropriate controls for flow cytometry experiment for determining MSCs surface markers?

I wish to characterize adipose stem cells (ADSCs) using flow cytometry. We have these antibodies: FITC-CD 73, FITC-CD 90, PE-CD105, FITC-CD 34, FITC-CD 45 and PE-HLA-DR. In general, the protocol I...

10 March 2023 8,698 3 View

How to culture adipose derived stem cells?

Hi, I'm facing a lot of difficulties in the culture of adipose derived stem cells (ADSCs). I use tissue explant method for the isolation of ADSCs; generally the cells start migrating out of the...

30 June 2018 9,941 10 View

How to process bone tissue for long term storage?

Hello all, I'm working with rabbit tibial defect model. After the completion of follow up time period, I'm gonna extract tibia from the animal and store for an undefined period of time. The bone...

31 August 2017 7,281 9 View

How to isolate cells from scaffold for MTT assay?

Hello all, I'm working with adipose stem cells and their potential in bone regeneration using different scaffolds. Can anyone suggest me a method to isolate ADSCs seeded onto a scaffold ( such as...

30 July 2017 5,729 8 View

Hello, can anyone provide me the detailed protocol for preparing scaffolds for bone regeneration?

Hello all, i'm working with mesenchymal stem cells and we're exploring the potential of MSCs in bone regeneration in a rabbit model via tissue engineering. So we have got chitosan powder but i...

25 July 2017 2,514 7 View