10 April 2014 1 9K Report

I read several papers and I found contrasting information regarding the expression of E-cadherin in HEK293 cells. I haven't run a WB as yet, but any personal experience will help.

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Scanning/analyzing zooplankton samples using a generic scanner?

Hello fellow scientists, Does anybody use a generic scanner to scan zooplankton samples? If so, what do you guys use in terms of software for segmentation of the original images into vignetes...

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We can found choaotic behavior in Biological Models Type SEIR, SEAIR, etc?

Dears, I am interested in chaotic behavior in classical biological models to spread any disease. To the best of my knowledge, I do not be able to find references that analyzed possible chaotic...

10 March 2023 7,281 5 View

Can anyone help me with Humberstone stain method for the simultaneous demonstration of Gram-positive and Gram-negative microorganisms in tissues?

Can anyone help me with Humberstone stain method for the simultaneous demonstration of Gram-positive and Gram-negative micro-organisms in paraffin sections?

21 December 2022 7,787 5 View

How to improve immunohistochemical staining in testis fixed with 4% PFA + 1.5% glutaraldehyde?

I am using antigen retrieval in citric acid (pH 6.0), followed by peroxidase blockade in H2O2+methanol and then serum blocking.

05 November 2021 5,687 2 View

Safe ambulation with femoral catheter?

how safe is it for routine ambulation if a patient has a femoral central line.? when would be safe enough to ambulate a pt. with a femoral central line

04 March 2021 7,006 3 View

Why my Chitosan (Medium Molecular Weight) sample is not soluble in HCl 2M but soluble in 2% Acetic acid?

I don't understand why my Chitosan sample (Sigma Aldrich Medium Molecular weight) is not soluble in HCl 2M solution (pH 1) but soluble in 2% Acetic acid solution (pH 4). For a hydrolisis protocol...

24 January 2021 2,096 3 View

How to separate large amounts of two Riboflavin-based compounds with similar solubilities?

I'm running a reaction in where riboflavin is derivatized with a crosslinker in DMSO. The final reaction mixture contains riboflavin (non reacted) and riboflavin-crosslinker compound (slightly...

13 December 2020 5,036 3 View

Why does my Phospho-4E-BP1 (Thr37/46) Antibody bind between 100-150kDa and not 15-20kDa?

Hi everyone. So I have tried to optimize my phospho-antibodies for western Blotting, however after several attempts the one antibody, Phospho-4E-BP1, keeps binding to the wrong protein. The...

19 October 2020 3,947 2 View

Are there studies (dyes or confocal laser scanning microscopy) on depth of penetration of citric acid into dentinal tubules?

are there studies (dyes or confocal laser scanning microscopy) on depth of penetration of citric acid into dentinal tubules?

12 October 2020 9,102 1 View

Covalent coupling IgG to HPR using HPR glycan and IgG primary amine?

I am looking for detailed and simple protocol to couple IgG with HPR using HPR glycans and IgG amine using periodate reaction. Best if no dyalisis is required. I plann to use antibodies attached...

07 August 2020 9,499 0 View

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