CRISPR/cas9
I want to find the genes related to development of female insects. I have the transcriptomic data, How I find? which pathways are related to development? Your consideration will be highly appreciated.
06 July 2018 663 0 View
I run the standard curve to know the primers specificity for amplification. I got this curve and melting peak. kindly explain whether it is right?
04 May 2018 8,349 0 View
I designed the primers for qRT PCR, but doing standard curve it shows the primers are not gene specific. I tried NCBI and other soft wares too. any suggestion please.
04 May 2018 2,685 0 View
I run the qRT PCR to detect the target gene expression with different time points. each sample have three technical replicates and compared with control. Now How I calculate the value of cq? how...
03 April 2018 9,223 2 View
I want to observe the larval and pupal stage of moth, for this rear them on artificial diet. Your kind help will be appreciated.
03 April 2018 1,573 2 View
We treated the adult moths and observed the egg hatching which was significantly reduced compared with control but the mating behavior was not changed. If we want to study the mechanism from...
01 January 1970 9,224 1 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Why knockouts for same genes from two different gRNA are showing different result? I followed CRISPR-CAS9 gene editing technology.
25 July 2024 8,313 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View
