CRISPR/cas9
I want to find the genes related to development of female insects. I have the transcriptomic data, How I find? which pathways are related to development? Your consideration will be highly appreciated.
06 July 2018 526 0 View
I run the standard curve to know the primers specificity for amplification. I got this curve and melting peak. kindly explain whether it is right?
04 May 2018 8,223 0 View
I designed the primers for qRT PCR, but doing standard curve it shows the primers are not gene specific. I tried NCBI and other soft wares too. any suggestion please.
04 May 2018 2,585 0 View
I run the qRT PCR to detect the target gene expression with different time points. each sample have three technical replicates and compared with control. Now How I calculate the value of cq? how...
03 April 2018 9,141 2 View
I want to observe the larval and pupal stage of moth, for this rear them on artificial diet. Your kind help will be appreciated.
03 April 2018 1,465 2 View
We treated the adult moths and observed the egg hatching which was significantly reduced compared with control but the mating behavior was not changed. If we want to study the mechanism from...
01 January 1970 9,146 1 View
Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...
01 March 2021 7,168 9 View
Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...
01 March 2021 1,843 2 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?
28 February 2021 8,095 1 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
The caterpillars of this moth were found in stems of Echinochloa polystachia grass as stem borers. The length of the caterpillars are something about 1.7 cm. I have compared images from the...
24 February 2021 309 3 View
The caterpillars of the moths were collected as fruitborers in pepper at a farmer. I reared them till the adults hatched and compared them with pictures and descriptions on the internet. Are these...
24 February 2021 7,785 5 View
I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...
23 February 2021 8,969 1 View
I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...
23 February 2021 9,007 3 View
This insect is minute in size around 1 mm and not clearly visible with naked eye.
22 February 2021 5,907 11 View
