I want to find the genes related to development of female insects. I have the transcriptomic data, How I find? which pathways are related to development?
Your consideration will be highly appreciated.
CRISPR/cas9
10 November 2018 2,050 0 View
I run the standard curve to know the primers specificity for amplification. I got this curve and melting peak. kindly explain whether it is right?
04 May 2018 8,349 0 View
I designed the primers for qRT PCR, but doing standard curve it shows the primers are not gene specific. I tried NCBI and other soft wares too. any suggestion please.
04 May 2018 2,685 0 View
I run the qRT PCR to detect the target gene expression with different time points. each sample have three technical replicates and compared with control. Now How I calculate the value of cq? how...
03 April 2018 9,223 2 View
I want to observe the larval and pupal stage of moth, for this rear them on artificial diet. Your kind help will be appreciated.
03 April 2018 1,573 2 View
We treated the adult moths and observed the egg hatching which was significantly reduced compared with control but the mating behavior was not changed. If we want to study the mechanism from...
01 January 1970 9,224 1 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...
24 July 2024 5,789 0 View
I need help for find a subject for PhD in insect physiology
12 July 2024 8,227 1 View
I want to know what are the relationship that happen between insect biodiversity and durian tree
10 July 2024 8,230 1 View
Hello everyone, I am currently working on a project involving spatial transcriptomics on E14 mouse embryos. We need to perform cryosectioning without fixing the embryos. Here is the protocol we...
10 July 2024 5,985 0 View
I need more information about this topic
27 June 2024 4,057 3 View
Hello, From my (naive) understanding, there are several target genes of the canonical Wnt signaling pathway that serve as reliable/validated transcriptional readouts of pathway activity. These...
18 June 2024 4,699 0 View
I am trying to estimate ROS in insect tissue. Is it possible to do it using DCFDA
18 June 2024 6,954 0 View
I'm a student of biological sciences and I need to collect information about how ambient temperature affects insects behaviour to answer the question: " which insects are more active in...
15 June 2024 367 3 View
I know that all animals and insects produces hormones. Do plants produce hormones that can have an equivalent effect on an animal like animal hormones?
13 June 2024 3,860 1 View