How to calculate the value of cq of qRT RCR for relative gene expression?

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Is CRISPR/CAS9 method is applicable In insect Moths for gene silencing?

CRISPR/cas9

10 November 2018 1,951 0 View

How to find female insect development related genes from a transcriptome?

I want to find the genes related to development of female insects. I have the transcriptomic data, How I find? which pathways are related to development? Your consideration will be highly appreciated.

06 July 2018 526 0 View

Please explain whether the primers are right for qRT PCR expression?

I run the standard curve to know the primers specificity for amplification. I got this curve and melting peak. kindly explain whether it is right?

04 May 2018 8,223 0 View

How to design the gene specific primers for qRT PCR?

I designed the primers for qRT PCR, but doing standard curve it shows the primers are not gene specific. I tried NCBI and other soft wares too. any suggestion please.

04 May 2018 2,585 0 View

Please suggest artificial diet for grain moths larvae?

I want to observe the larval and pupal stage of moth, for this rear them on artificial diet. Your kind help will be appreciated.

03 April 2018 1,465 2 View

Which Endocrine signaling Pathway effects on Insect oviposition?

We treated the adult moths and observed the egg hatching which was significantly reduced compared with control but the mating behavior was not changed. If we want to study the mechanism from...

01 January 1970 9,146 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Adil Hussain

Sakhawat Badhsha, I am sure you'll have an attached computer system with the qPCR machine. The computer system comes equipped with the relevant software compatible with the qPCR system. It will do everything for you. You can just get the final/analyzed data from the computer.

Sakhawat Shah

Thanks Sir for Your kind guidance.