Sakhawat Shah

7 Questions 3 Answers 0 Followers

Questions related from Sakhawat Shah

Is CRISPR/CAS9 method is applicable In insect Moths for gene silencing?

CRISPR/cas9

11 November 2018 2,016 0 View

How to find female insect development related genes from a transcriptome?

I want to find the genes related to development of female insects. I have the transcriptomic data, How I find? which pathways are related to development? Your consideration will be highly appreciated.

07 July 2018 606 0 View

Please explain whether the primers are right for qRT PCR expression?

I run the standard curve to know the primers specificity for amplification. I got this curve and melting peak. kindly explain whether it is right?

05 May 2018 8,275 0 View

How to design the gene specific primers for qRT PCR?

I designed the primers for qRT PCR, but doing standard curve it shows the primers are not gene specific. I tried NCBI and other soft wares too. any suggestion please.

05 May 2018 2,651 0 View

How to calculate the value of cq of qRT RCR for relative gene expression?

I run the qRT PCR to detect the target gene expression with different time points. each sample have three technical replicates and compared with control. Now How I calculate the value of cq? how...

04 April 2018 9,194 2 View

Please suggest artificial diet for grain moths larvae?

I want to observe the larval and pupal stage of moth, for this rear them on artificial diet. Your kind help will be appreciated.

04 April 2018 1,527 2 View

Which Endocrine signaling Pathway effects on Insect oviposition?

We treated the adult moths and observed the egg hatching which was significantly reduced compared with control but the mating behavior was not changed. If we want to study the mechanism from...

01 January 1970 9,199 1 View