I run the standard curve to know the primers specificity for amplification. I got this curve and melting peak. kindly explain whether it is right?
CRISPR/cas9
10 November 2018 1,951 0 View
I want to find the genes related to development of female insects. I have the transcriptomic data, How I find? which pathways are related to development? Your consideration will be highly appreciated.
06 July 2018 526 0 View
I designed the primers for qRT PCR, but doing standard curve it shows the primers are not gene specific. I tried NCBI and other soft wares too. any suggestion please.
04 May 2018 2,585 0 View
I run the qRT PCR to detect the target gene expression with different time points. each sample have three technical replicates and compared with control. Now How I calculate the value of cq? how...
03 April 2018 9,141 2 View
I want to observe the larval and pupal stage of moth, for this rear them on artificial diet. Your kind help will be appreciated.
03 April 2018 1,465 2 View
We treated the adult moths and observed the egg hatching which was significantly reduced compared with control but the mating behavior was not changed. If we want to study the mechanism from...
01 January 1970 9,146 1 View
I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.
03 March 2021 5,997 2 View
Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...
03 March 2021 2,350 2 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.
02 March 2021 4,359 3 View
Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...
02 March 2021 7,829 3 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...
01 March 2021 8,235 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...
01 March 2021 4,128 3 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View