Hello,
I usually keep my liquid media Luria Broth, YPD and YES in laminar air flow (LAF), after my work is done. So while I sterilise LAF with U.V light, does it effect my media?
Yes, it does affect the media. There is a very old research article that tested the properties of UV irradiation on yeast culture media and found that it induces toxicity (probably by affecting the sugars and amino acids).
A recent study conducted similar experiments and found that UV impacted the chemical composition of cell culture media as well. However, the use of such media did not affect the growth of Chinese Hamster Ovary cells. Please find attached both articles.
Hi there,
Molecules absorbing UV can be subjected to molecular rearrangment/modification. Consequently the composition of the media exposed to UV might be modified.
As answered by previous guys, yes UV may affect the medium composition. just one short example: yeast media are frequently containing tryptophane (added in some synthetic media or included in the powders in rich one). Tryptophane will react with string UVs to gives tryptophan dimers with two cosnequences: the tryptophane concentration of the culture medium will decrease, and the tryptophane dimers are toxic for yeast cells. SO, Yaseen take care !
Philippe
I agree with the explanation given by earlier distinguished members of the Research Gate. It is, therefore, imperative that the yeast culture media should not be exposed to UV irradiation.We are working on the yeast culture since 1973 and we never exposed our cultures to UV irradiation.
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