Is Arabidopsis U6 promoter will work in rice or other monocot cereal? Please share your expariance.......if worked with Arabidopsis u6 promoter in monocot plants
Example- If Arabidopsis U6 promoter will work in Rice/or Brachipodium
I am not sure whether anyone has done this kind of experiments where AtU6 promoter was used to drive a gene in rice or other monocots.
However, from this company's website (see below link), it seems as if they prefer to use AtU6 in dicots for CRISPR. This is what they say: "RNA is driven by the U6 promoter from Arabidopsis thaliana (for dicots) or from Oryza sativa (for monocots)."
http://www.dna-cloning.com/
I cant tell you for sure. But, for generating overexpressor/ RNAi lines, I used xylem specific promoter from poplar in Arabidopsis and it worked well. But, I cant say for CRISPR constructs. May be worth going to literature.
If you want to find out, you can do a quick 'transient assay' by bombarding a AtU6::GUS construct into rice tissue. If you do so, don't forget to bombard the same construct into Arabidopsis plant tissues for control.
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