What should be the possible approach to detect the adjact site of formation of Reactive oxygen species.

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Is Arabidopsis U6 promoter will work in rice or other monocot cereal?

Is Arabidopsis U6 promoter will work in rice or other monocot cereal? Please share your expariance.......if worked with Arabidopsis u6 promoter in monocot plants

04 May 2016 7,617 4 View

Good software for DNA & RNA secondry structure prediction?

31 December 2015 7,882 9 View

How to determine the size of TAL protein?

Our TALEN nucleotide sequence is 3750 (ORF) and according to this our protein size should be approxinmately 137kda but size is coming around 75-100 kda. Any suggestions?

09 October 2013 5,274 1 View

How can we express and isolate talen protein?

We custom-designed talen activator sequence and it is in a vector. I need to express this protein and need to isolate it. I do not know the nature of protein. We have pet 45b expression vector t7...

07 August 2013 4,003 2 View

Which online software is good for the promoter prediction of a known sequence (upstream sequence + gene sequence)?

How accurate is the software?

04 May 2013 6,859 15 View

Which is the best online tool for microRNA analysis and target prediction?

MicroRNA degredome analysis.

03 April 2013 7,619 3 View

Which is the best online software (free) available for micro RNA target prediction?

MicroRNAs are a class of post-transcriptional regulators. They are short ~22 nucleotide RNA sequences that bind to complementary sequences in the 3’ UTR of multiple target mRNAs, usually resulting...

31 December 2012 9,427 5 View

What may be the possible strategies to increase the efficiency of embryogenesis from microspore in wheat/triticle?

The percentage of formation of embryo and then green plants is low. We need to increase the efficiency.

10 November 2012 7,735 1 View

How can MGD (N-methyl-D-glucamine dithiocarbamate) be synthesized using N-methyl-D-glucamine and carbon disulfide?

MGD used for detection of nitric oxide radicals

08 September 2012 6,033 0 View

What are the possible strategies to increase photosynthetic efficiency?

Photosynthetic efficiency.

08 September 2012 8,725 11 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View