Are you interested in the prediction of promoter regions or just the prediction of cis-regulatory elements? For the former, you can check this compilation of resources: http://www.shodhaka.com/cgi-bin/startbioinfo/simpleresources.pl?tn=Promoter%20prediction

Of course this prediction has its limitations, since not all promoters in eucaryotes have the same characteristic elements (for example, not all of them have TATA box), but some of the limitations are mentioned in the compilation.

If looking for cis-regulatory elements in a promoter region, you can use the databases mentioned by Daniel, and also give a try to Transfac http://www.gene-regulation.com/pub/databases.html or PLACE, if working with plant sequences http://www.dna.affrc.go.jp/PLACE/

However, all these databases just look for regulatory elements that have already been reported in a particular promoter in response to a particular stimulus. However, transcription factors can sometimes be promiscuous and are able to bind slightly different sequences, or, when the element is in combination with another element, sequence restrictions can change. In these cases maybe the search for already known elements won't be very useful.

For de novo prediction of regulatory elements, I recommend using (if available) sequences of orthologous promoters or promoters of genes co-expressed with your gene of interest. In those cases you would expect they share functional regulatory elements that are evolutionarily conserved. Having those sequences you can use SCOPE http://genie.dartmouth.edu/scope/ or Melina http://www.openhelix.com/cgi/tutorialInfo.cgi?id=26

Good luck!

Try the mammalian promoter database, http://mpromdb.wistar.upenn.edu/

the eukaryotic promoter database, http://epd.vital-it.ch/

or the Transcriptional Regulatory Element Database, http://rulai.cshl.edu/cgi-bin/TRED/tred.cgi?process=home

If you are working with a non-mammalian species, the resources would change, so please make mention of it.

The major limitation of these databases is that in addition to proximal promoters, enhancers can also play a major role in gene regulation, and it is not trivial to map them to your gene of interest. Nonetheless, these databases can be a good starting point for reporter assays or for looking at potential motifs/TFs that might regulate a target gene of interest.

Can you make your question more specific, what do you need the promoter sequence for? And what species Mammalian ? or plant?

Are you interested in the prediction of promoter regions or just the prediction of cis-regulatory elements? For the former, you can check this compilation of resources: http://www.shodhaka.com/cgi-bin/startbioinfo/simpleresources.pl?tn=Promoter%20prediction

Of course this prediction has its limitations, since not all promoters in eucaryotes have the same characteristic elements (for example, not all of them have TATA box), but some of the limitations are mentioned in the compilation.

If looking for cis-regulatory elements in a promoter region, you can use the databases mentioned by Daniel, and also give a try to Transfac http://www.gene-regulation.com/pub/databases.html or PLACE, if working with plant sequences http://www.dna.affrc.go.jp/PLACE/

However, all these databases just look for regulatory elements that have already been reported in a particular promoter in response to a particular stimulus. However, transcription factors can sometimes be promiscuous and are able to bind slightly different sequences, or, when the element is in combination with another element, sequence restrictions can change. In these cases maybe the search for already known elements won't be very useful.

For de novo prediction of regulatory elements, I recommend using (if available) sequences of orthologous promoters or promoters of genes co-expressed with your gene of interest. In those cases you would expect they share functional regulatory elements that are evolutionarily conserved. Having those sequences you can use SCOPE http://genie.dartmouth.edu/scope/ or Melina http://www.openhelix.com/cgi/tutorialInfo.cgi?id=26

Good luck!

I need for plant in wheat, rkd gene promoter sequance (tarkd1 and tarkd2)........

Try promoter analysis pipeline(PAP) or LASAGNA search..

Such as Promoter Scan, the others, you can view online:

http://www-bimas.cit.nih.gov/molbio/proscan/

http://www.fruitfly.org/seq_tools/promoter.html

http://www.cbs.dtu.dk/services/Promoter/

http://bioportal.bic.nus.edu.sg/tres/

SCOPE

Expasy

www.expasy.org/

you can try www.cisexpress.org

try www.genomatix.de/matinspector ; this software is free. More complex softwares provided by genomatix allow the comparison between species, for example "eldorado" but you should buy them

I am looking for Known TF binding sites in mammalian cells in my candidate genes.

Whats the best input sequence for data bases like NHR scan or JASPAR?

Is is possible to choose the promoter area by the http://mpromdb.wistar.upenn.edu

and then map it for the TF binding site?

Try this:

http://www.sabiosciences.com/chipqpcrsearch.php?app=TFBS

Then, you can use other more specific as: JASPAR or PROMO

PLEASE send me promoter region sequence upto 1kb upstream of bovine TLR4 gene. also send me the online tool for extracting the sequence.

please help me

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