Is anyone familiar with the protocol for developing antibody against pseudomonas in rabbit?

More Narayanaswamy Krithiga's questions See All

How much tissue shrinkage does 4% PFA post perfusion cause?

How much shrinkage can I expect after fixing mouse brain with 4% PFA? Would 10% be reasonable or 20? Thank you!

29 August 2021 3,291 1 View

I want to distinguish delta ferrite from normal ferrite in microstructure. can anyone suggest a solution pl ?

how to differentiate in a microstructure , the difference between Ferrite & delta ferrite which occurs in phosphate case hardened structures

27 April 2017 7,804 4 View

Can anyone suggest me, which is the most suitale method to trasfect micro RNA mimic into HeLa cells?

I am planing to study the effects of micro RNA on protein expression levels in HeLa cells. Which is the most preferable method for transfection of micro RNA mimic? Any recommendation for the...

09 October 2016 2,927 2 View

How can i see whether there is any interference with heavy metals for enzymes ?

for example how can i see whether the polymer coated nanoparticles interact with the enzyme pls suggest me some assays

15 August 2016 4,677 1 View

Can anyone suggest some ways to cause gastric cancer via cell line or chemical induced in invivo mice models?

Can anyone suggest some ways to cause gastric cancer via cell line or chemical induced in invivo mice models

26 January 2016 9,541 2 View

Where we can order cancer aptamers?

Sensing with the help of aptamers.

10 November 2015 1,979 3 View

Can we determine inhibition effect of the divalent or monovalent ions to the enzymes?

enzyme inhibition studies for divalent or monovalent ions can be studied using the same enzyme assay or with a different one?

01 January 1970 288 3 View

How we can solve system of non linear PDE in Mathematica, I'm checking Mathematica package of Bvph2.0 I'm not getting output from this ?

i have attached some problem i m trying to do with Mathematica HAM for this kind equations

01 January 1970 1,430 2 View

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

12 August 2024 862 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View