How much shrinkage can I expect after fixing mouse brain with 4% PFA? Would 10% be reasonable or 20? Thank you!
Ther is only minimal shrinkage after perfusin if you work later with non embedded tissue. If you embed the tissue in paraffin or other resins where you need an alcoholic dehydration then you can expect 10 -20 % of shrinkage
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...
03 March 2021 2,297 3 View
03 March 2021 8,272 1 View
Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...
03 March 2021 2,037 3 View
Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...
03 March 2021 6,317 1 View
i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).
03 March 2021 7,100 1 View
03 March 2021 5,360 2 View
The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...
03 March 2021 4,107 4 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
02 March 2021 5,204 3 View
How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...
01 March 2021 2,294 3 View
Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!
01 March 2021 9,574 4 View
I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples
22 February 2021 1,328 1 View
Hi, i am conducting a Monte Carlo simulation for my new distribution but the MSE values are increasing while the sample size increase, does any experience this issue before and what should i do...
22 February 2021 3,680 4 View
I need to take IF images with DAPI on a confocal but I need to use a glycerol fixation. Has anyone done this method before, if so how was it done? Thank you.
21 February 2021 3,081 3 View
I have some fixed slides of tissue and need to do some staining, but I am not sure where to start. There are many protocols online. Any suggestions?
17 February 2021 3,062 4 View
I am currently trying to copy an assembly feature, that is present in an old version of the model I am using, onto a new model. The feature is a reference point that is fixed to an attachment...
16 February 2021 8,872 2 View
15 February 2021 911 5 View
I am embedding PFA fixed tissues in low melting point agarose prior to sectioning and I am wondering how long can these can be kept whilst maintaining tissue integrity and fluorescent signal?...
14 February 2021 6,688 3 View
I have a very thin sample, the thickness is around 1 mm. I want to do X-ray diffraction measurement on the thin side of the sample. What kind of tape can I use to fix the sample, and will not...
13 February 2021 4,153 3 View