Is anybody working with DNA methylation using MSAP?

14 August 2014 1 9K Report

I faced problem to stain and visualize denaturing PAGE gel. I will appreciate if you share your practical experience in this issue.

Pranay Amruth Maroju

Dear Juran

I hope you are following post run staining protocol where you remove your PAGE gel from cassette and release it into a 100ml TAE buffer containing 7-10ul EtBr and put it on a rocker for 10 min and then wash it with 100ml of TAE buffer without EtBr and then look on a UV Transilluminator. This method works fine.

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