Why total proteins (Eg Akt PI3K) are similar in all groups ?

I read several article they indicated total protein like PI3K Akt IKKbeta etc values are similar in control, inducer, treatment. Kindly explain

01 February 2016 9,465 2 View

Which is a better vehicle for anticancer drug delivery: soybean oil or corn oil?

My anticancer drug does not dissolve in water and DMSO hence I chose a vehicle oil for oral drug delivery of my rat.

11 December 2013 4,192 2 View

Is a dialysis tube suitable for protein purification?

I have a ready-to-work protein purification method for a western blot analysis. Here, the dialysis tube method is a very simple method for protein purification. My doubt is whether dialysis...

08 September 2013 6,268 1 View

Can anyone suggest a protocol to extract proteins from buccal tissue?

Isolation of proteins from buccal tissue is challenging because it has very rough content and its protein amount is very low.

08 September 2013 3,783 1 View

Preparative hplc surely separate all compound from crude extract

If preparative hplc can separate individual compound from crude extract if yes how to we isolate that compound. Kindly help me

09 October 2012 2,752 3 View

Are there any new antigens recently identified in oral cancer?

Oral cancer antigens

08 September 2012 373 1 View

Is DNA minor groove binder an anticancer agent?

Why are all anticancer agent binding to DNA minor groove?

02 March 2012 7,393 0 View

Different between plant cytochrome p450 and animal cytochrome p450

What was the duty of cytochrome p450 in plant and animal cell

01 February 2012 7,871 0 View

Why antioxidant enzyme and non-enzyme antioxidant activity were differ from plant and animal

Hello friends I am watching some article entitle antioxidant against algal bloom. In this articles maximizes antioxidant were decrease SOD , CAT and GPx and increase Vit-E, Vit-C and GSH. But in...

01 February 2012 5,880 0 View

need Molecular technique in oral cancer

Hi i need molecular technique for oral cancer. If any one know kindly send please. I will need this technique for send my proposal.

31 December 2011 8,339 4 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View