For measuring SOD, Cat, GPx, GSH, Vit C and E.
I read several article they indicated total protein like PI3K Akt IKKbeta etc values are similar in control, inducer, treatment. Kindly explain
01 February 2016 9,566 2 View
My anticancer drug does not dissolve in water and DMSO hence I chose a vehicle oil for oral drug delivery of my rat.
11 December 2013 4,255 2 View
I have a ready-to-work protein purification method for a western blot analysis. Here, the dialysis tube method is a very simple method for protein purification. My doubt is whether dialysis...
08 September 2013 6,330 1 View
Isolation of proteins from buccal tissue is challenging because it has very rough content and its protein amount is very low.
08 September 2013 3,843 1 View
If preparative hplc can separate individual compound from crude extract if yes how to we isolate that compound. Kindly help me
09 October 2012 2,846 3 View
Oral cancer antigens
08 September 2012 429 1 View
Why are all anticancer agent binding to DNA minor groove?
02 March 2012 7,452 0 View
Hello friends I am watching some article entitle antioxidant against algal bloom. In this articles maximizes antioxidant were decrease SOD , CAT and GPx and increase Vit-E, Vit-C and GSH. But in...
01 February 2012 5,941 0 View
What was the duty of cytochrome p450 in plant and animal cell
01 February 2012 7,955 0 View
Hello in my doubt is "aflatoxin b1" can individually induce cancer without adding any promoter agent'. True or not
31 December 2011 3,541 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...
01 August 2024 5,460 5 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...
30 July 2024 2,483 5 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I did an MTT assay with 2 drugs (Piroxicam and Sulfameter) when I got my results for the 12,24,48,72 hours of incubation with my drug at the following concentrations [ug/ml]...
26 July 2024 7,511 3 View
I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...
23 July 2024 8,492 3 View
