Preparative hplc surely separate all compound from crude extract

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Why total proteins (Eg Akt PI3K) are similar in all groups ?

I read several article they indicated total protein like PI3K Akt IKKbeta etc values are similar in control, inducer, treatment. Kindly explain

01 February 2016 9,465 2 View

Which is a better vehicle for anticancer drug delivery: soybean oil or corn oil?

My anticancer drug does not dissolve in water and DMSO hence I chose a vehicle oil for oral drug delivery of my rat.

11 December 2013 4,192 2 View

Is a dialysis tube suitable for protein purification?

I have a ready-to-work protein purification method for a western blot analysis. Here, the dialysis tube method is a very simple method for protein purification. My doubt is whether dialysis...

08 September 2013 6,268 1 View

Can anyone suggest a protocol to extract proteins from buccal tissue?

Isolation of proteins from buccal tissue is challenging because it has very rough content and its protein amount is very low.

08 September 2013 3,783 1 View

Is any kit available for analysis of enzymatic and non-enzymatic antioxidants at low cost?

For measuring SOD, Cat, GPx, GSH, Vit C and E.

31 December 2012 637 0 View

Are there any new antigens recently identified in oral cancer?

Oral cancer antigens

08 September 2012 373 1 View

Is DNA minor groove binder an anticancer agent?

Why are all anticancer agent binding to DNA minor groove?

02 March 2012 7,393 0 View

Different between plant cytochrome p450 and animal cytochrome p450

What was the duty of cytochrome p450 in plant and animal cell

01 February 2012 7,871 0 View

Why antioxidant enzyme and non-enzyme antioxidant activity were differ from plant and animal

Hello friends I am watching some article entitle antioxidant against algal bloom. In this articles maximizes antioxidant were decrease SOD , CAT and GPx and increase Vit-E, Vit-C and GSH. But in...

01 February 2012 5,880 0 View

need Molecular technique in oral cancer

Hi i need molecular technique for oral cancer. If any one know kindly send please. I will need this technique for send my proposal.

31 December 2011 8,339 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

Any easy method of analyzing vitamin D in foods by HPLC?

Looking for method of extracting and analyzing vitamin D in foods by HPLC

24 February 2021 7,524 7 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View

Which vial septa the right one to be used for HPLC?

This is the unanswered question stored very long in my mind and till now I could not find a right answer. Google search educated me that the red surface is the PTFE material compatible to wider...

21 February 2021 546 3 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View