Isolation of proteins from buccal tissue is challenging because it has very rough content and its protein amount is very low.
Are you concerned with the proteins you will extract from all the microbes living in buccal tissue or are you particularly interested in human proteins?
I read several article they indicated total protein like PI3K Akt IKKbeta etc values are similar in control, inducer, treatment. Kindly explain
01 February 2016 9,566 2 View
My anticancer drug does not dissolve in water and DMSO hence I chose a vehicle oil for oral drug delivery of my rat.
11 December 2013 4,255 2 View
I have a ready-to-work protein purification method for a western blot analysis. Here, the dialysis tube method is a very simple method for protein purification. My doubt is whether dialysis...
08 September 2013 6,330 1 View
For measuring SOD, Cat, GPx, GSH, Vit C and E.
31 December 2012 712 0 View
If preparative hplc can separate individual compound from crude extract if yes how to we isolate that compound. Kindly help me
09 October 2012 2,846 3 View
Oral cancer antigens
08 September 2012 429 1 View
Why are all anticancer agent binding to DNA minor groove?
02 March 2012 7,452 0 View
Hello friends I am watching some article entitle antioxidant against algal bloom. In this articles maximizes antioxidant were decrease SOD , CAT and GPx and increase Vit-E, Vit-C and GSH. But in...
01 February 2012 5,941 0 View
What was the duty of cytochrome p450 in plant and animal cell
01 February 2012 7,955 0 View
Hello in my doubt is "aflatoxin b1" can individually induce cancer without adding any promoter agent'. True or not
31 December 2011 3,541 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View