Isolation of proteins from buccal tissue is challenging because it has very rough content and its protein amount is very low.
Are you concerned with the proteins you will extract from all the microbes living in buccal tissue or are you particularly interested in human proteins?
I read several article they indicated total protein like PI3K Akt IKKbeta etc values are similar in control, inducer, treatment. Kindly explain
01 February 2016 9,465 2 View
My anticancer drug does not dissolve in water and DMSO hence I chose a vehicle oil for oral drug delivery of my rat.
11 December 2013 4,192 2 View
I have a ready-to-work protein purification method for a western blot analysis. Here, the dialysis tube method is a very simple method for protein purification. My doubt is whether dialysis...
08 September 2013 6,268 1 View
For measuring SOD, Cat, GPx, GSH, Vit C and E.
31 December 2012 637 0 View
If preparative hplc can separate individual compound from crude extract if yes how to we isolate that compound. Kindly help me
09 October 2012 2,752 3 View
Oral cancer antigens
08 September 2012 373 1 View
Why are all anticancer agent binding to DNA minor groove?
02 March 2012 7,393 0 View
What was the duty of cytochrome p450 in plant and animal cell
01 February 2012 7,871 0 View
Hello friends I am watching some article entitle antioxidant against algal bloom. In this articles maximizes antioxidant were decrease SOD , CAT and GPx and increase Vit-E, Vit-C and GSH. But in...
01 February 2012 5,880 0 View
Hi i need molecular technique for oral cancer. If any one know kindly send please. I will need this technique for send my proposal.
31 December 2011 8,339 4 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...
25 February 2021 8,404 3 View
Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...
25 February 2021 1,660 3 View
I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?
25 February 2021 9,761 1 View
Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...
25 February 2021 3,612 3 View