Why does sodium metabisulfite generate turbidity when mixing with sodium hydroxide?

When I tried to mix a sodium metabisulfite solution with sodium hydroxide solution, the mixture solution quickly became turbid. Before mixing both were clear solutions. Please help explain the...

03 February 2021 8,075 2 View

Any suggestion for conductive paste for PDMS or Ecoflex for electrode?

Dear colleagues, I am trying to make a sturdy electrode on a flexible polymer substrate (PDMS or Ecoflex). I have used a silver paste, but once cured, it peels off very easily. the cured silver...

14 December 2020 4,236 3 View

Are there any easy way to test the activity of CYP51?

I'm looking for a relative easy way to test the activity of CYP51. Are there any way that I can measure it without using LC-MS or gas chromatography. A simple absorbance or fluorescence test will...

08 October 2020 6,687 3 View

Can we use HR-ICP-MS to measure As?

Hi there, we have the ICPMS element 2 using argon gas. I am wondering whether we can use element 2 to measure arsenic? Can you please provide more information or papers that I can check? Thanks!

16 August 2020 1,142 3 View

Where to publish systematic review protocol?

I would like to prepare a systematic review protocol. I am wondering about any journals that will publish a protocol without charges from the authors? It does not have to be open access. The topic...

23 July 2020 4,537 8 View

How to perform western blot for Toll-like receptor 3?

I encountered some problem to detect TLR3 for my western. I did not get any bands. After reading a bunch of literature, I am not sure exactly what I did was wrong. I appreciate if anyone has...

19 July 2020 9,402 3 View

How to minimize non-specific binding of eGFP protein to magnetic nanoparticles?

I am working on protein conjugation into nanoparticles. Magnetic nanoparticles were washed with DI water and TBS buffer and then combined with eGFP fused protein for 24 hrs. After removing the...

01 July 2020 8,864 3 View

Where to find recovery rate and death rate of COVID-19?

Some people, even doctors say it's not a serious disease, it's like seasonal flu, which could cause death. Where to find the recovery rate and death rate of COVID-19 in respect of older adults...

16 June 2020 6,546 12 View

Can we only capture one diffraction order in light modulator?

Obviously, the spatial light modulator (SLM) and digital micromirror device (DMD) is diffration gratings. Therefore there would be multiple diffraction orders of the created images. Here I want...

06 May 2020 396 8 View

If the cofactor(NAD) concentration in 1mM and substrate is 10mM in enzyme reaction, the substrate become 9mM after reaction?

If the cofactor(NAD) concentration in 1mM and substrate is 10mM in enzyme reaction, the substrate become 9mM after reaction?

02 March 2020 3,982 6 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

How to get started building and analyzing a UTAUT model?

Hello all, I need help getting started building and analyzing a UTATU model. I have a survey ready with some questions but I had some confusions on UATAUT and on how to analyze the data. I will...

02 March 2021 9,271 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Could anyone tell me what is the range of submillimeter wave?

There is confusion regarding the submillimeter-wave range. Many papers specify its range from 300GHz but some also consider its range from 100GHz. If I design an antenna on 122ghz what would I...

01 March 2021 516 7 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

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22 February 2021 9,932 11 View

Which type of loading is to be used in dynamic analysis of Machien foundation?

Hello Everyone, Hope you all are doing well. I'm trying to simulate a machine foundation in ABAQUS with a geo-cell reinforced soil bed. Im totally confused to which type of loading would be...

21 February 2021 4,388 3 View