The first and most simple approach will be the elimination or repression of repressive gene functions for abiotic and biotic stresses. In particular in cases where there a more gene copies due to a higher complex genomes like wheat or other duplications that hinder or even make it impossible to achieve the goal by conventional breeding. A prominent example, which is already achieved and probably on the fields is the addressing of 6 MLO copies in the wheat genome. Their elimination was achieved by CRISPR and also earlier by TALEN. It also demonstrated problems with side effects (penalty) which, however, also could be overcome: see Article Slicing the cost of bread
One area where CRISPR-Cas is proving to be highly effective in plant breeding is in developing disease-resistant crops. By targeting specific genes responsible for susceptibility to diseases, researchers can use CRISPR-Cas to introduce precise genetic modifications that enhance the plant's natural defense mechanisms. This approach has the potential to significantly reduce crop losses due to diseases, ultimately leading to improved yields and food security. Additionally, CRISPR-Cas can also be employed in enhancing nutritional content or improving stress tolerance in plants , further contributing to the overall improvement of crop quality. By manipulating specific genes involved in nutrient synthesis or stress response pathways, scientists can use CRISPR-Cas to create crops with higher levels of essential vitamins and minerals, making them more nutritious for human consumption. Furthermore, by enhancing the plant's ability to withstand harsh environmental conditions, such as drought or extreme temperatures, CRISPR-Cas technology can help ensure stable and reliable crop production, even in challenging circumstances. Overall, the versatility of CRISPR-Cas offers immense potential for revolutionizing agriculture and addressing global food security concerns.
it is a powerful tool that, when used responsibly, can contribute significantly to the future of agriculture by creating crops that are more resilient, nutritious, and sustainable.
CRISPR-CAS technology has opened the door to new possibilities in basic research, plant breeding, genetic engineering and genome editing. Through genome editing using CRISPR CAS technology is possible to develop crop varieties with desirable traits such as high yielding and disease resistant crop varieties. At present, genome editing of various plants and animals is being done by using this technology in biotechnological laboratories in various European countries, including China America, Japan and India. This technology is even being used in the treatment of various human diseases. The successful use of this technology in improving various traits including increasing the yield of crops such as rice, wheat, maize, barley, potato, tomato etc. is promising.
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