We want to test a drug on mice and will inject it intraperitonealy. Our problem is that the drug is deactivated if diluted in saline or PBS. I read that distilled water cannot be used. What would you recommend?
Hello, I would like to know if someone could advice me about the best device to use to measure artificial light at night in coastal areas, outside and inside water (just few meters)? Thank you...
23 April 2024 8,319 1 View
I am pursuing my PhD and chose the topic of pros/cons and ethical concerns in leveraging Artificial Intelligence tools to increase Equity in Performance Management/Appraisal and did not find much...
18 November 2023 8,460 2 View
I want to calculate the ID/IG ratio from a carbon sample measured by Raman. After obtaining the spectra, I have deconvoluted D and G peaks by fitting them with 2 Gaussian-Lorentzian curves using...
16 November 2023 6,458 1 View
Hello Can I put a html file (a network graph) ?
08 June 2023 613 1 View
I want to know how much organic carbon is a good percentage in a coffee crop, having in consideration that it will be taken from the first 30 centimeters of the sampling point.
14 May 2023 7,638 2 View
I have contingency table with 2 treatments (t1, t2) and 3 stages (s1, s2, s3) as following: T1 T2 s1 x1 x2 s2 y1 y2 s3 z1 z2 I got a significant interaction when i did...
18 January 2023 8,942 4 View
Currently I'm struggling with choosing between various analysis options, ranging from repeated measures design, to 4-way ANOVA to ANCOVA or to moderation analysis with the PROCESS macro of Hayes....
17 January 2023 1,628 4 View
Hi, I need to compare the alpha diversity of three communities (the gut bacteria; the gastro-intestinal helminth community ; the bacteria community found in the spleen of rodents) For the gut...
29 September 2022 4,851 2 View
I have some polar transformed data that I would like to represent in a graph. I was used to do that in Statistica; however, I cannot find a way to do it in SPSS. Does someone know if/how to do it...
28 March 2022 5,436 3 View
For one of my studies, I am using Q methodology for the first time. I am currently developing the Q-survey that will be administered to participants on an online platform (I am considering using...
14 March 2021 8,837 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View