We used rat total blood using EDTA as anticoagulante. For EPC's identification the CD34, CD133, CD146 and KDR were used.
Are you asking about other CD45+ cells in your sample? Or could you’re EPC's also express CD45?
CD45 is a marker expressed and shared by all Immune cells so of course you might find lots of positive cells in your sample.
I don’t know about EPC's, but since they are not of the hematopoietic lineage, it would be pretty interesting if they do express it.
Just add anti-CD45 to your flow panel and run your sample. And you'll find the answer to your question.
Thank you both for your contribution to this subject.
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