In IHC for double staining i use DAB and AP. DAB works fine but AP after mounting is a headache as i see precipitates and also sometimes dissolves.I have tried drying the slides a bit and then mounting with the aqueous mounting media. I first wash it with water or rather rinse it with water after NBT-BCIP step and then mount.
How do i overcome this.
Thanks in advance.
Naren
I do not dry the slides. I use Vector Vectamount Permanent Mounting Medium. I just remove the slides from the CitriSolv or other non xylene solvent, tap to remove as much excess as possible and apply mounting medium. It should not interfere with your chromagen staining. What mounting medium are you using?
Renee R Donahue once i develop with the chromagen NBT-BCIP, i rinse with water and take out the excess water and mount with aqueous mounting medium that i bought from sigma.
sometimes i get these precipitates or sometimes the developed stain dissolves.
any tips?
thanks again
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