Narendran Reguraman

5 Questions 11 Answers 0 Followers

Questions related from Narendran Reguraman

Cytoplasmic staining of BCL-6 in spleen tissue of treated groups rather than nuclear?

I have 3 groups of animal apart from PBS controls where i treat them under different immunosuppressive conditions. when the animals are subjected to partial or no immunosuppressive conditions, i...

01 September 2020 9,142 5 View

In IHC - Alkaline phosphatase method, do we dry the slides or not before mounting in aqueous medium?

In IHC for double staining i use DAB and AP. DAB works fine but AP after mounting is a headache as i see precipitates and also sometimes dissolves.I have tried drying the slides a bit and then...

27 July 2020 370 2 View

How do i use a rabbit monoclonal antibody on rabbit tissues with minimal or no background staining?

I am working with rabbit spleen and as it is difficult to obtain antibodies against rabbits as most of them are raised in rabbits. I had to purchase a few antibodies raised in rabbits. But as...

18 June 2020 4,047 8 View

Background fluorescent staining through different?

I am trying stain for two membrane proteins, one cellular and one viral. But lately, i am doubting a little that the double positive staining could be because of background staining of the other...

20 November 2019 9,724 3 View

I am facing difficulty in immunofluorescence due to autofluorescence?

I am trying out immunofluorescence on rabbit spleen but the results are not fruitful due to auto-fluorescence. I tried blocking with 5%BSA for an hour still persists. I also tried sudan black but...

21 March 2019 1,715 8 View