Hi Siddharth Narendran ,

You can use the NEB LAMP Primer Design Tool (https://lamp.neb.com/#!/)

Also, the file I attached can be helpful.

You can use LAMP Designer http://www.premierbiosoft.com/jsp/customer/RequestEvaluation.jsp

Dear Narendran, if I got well your question, firstly, you might probably willing to design universal LAMP primers for one conserved sequence which is found across the six different fungal species. Secondly, you may be looking for designing the primers that are only specific for each single fungal strain to avoid cross-reactivity upon detection. Therefore, in both scenarios you will have to use NCBI for nucleotide BLAST to find conserved regions among your fungal species. After getting your sequences of interest from NCBI, I suggest you to go for PREMIER Biosoft LAMP Designer (http://www.premierbiosoft.com/jsp/customer/RequestEvaluation.jsp) as recommended by Ghorbani because it is faster, convenient, and generate all the LAMP primers including Loop primers at once. You can apply for a free trial before you decide to buy, and follow a demo video to learn how to use the product in the link (http://www.premierbiosoft.com/isothermal/index.html). PrimerExplorer V4 or V5 versions can also be used but they sometimes generate only 4 primers without loop primers, or LB only without LF. At the end, you end up designing loop primers separately or manipulating certain parameters such as length of your target sequence, TM, …..

Hope this will help!

Good luck!