Please help me to find out research centers in India providing Inelastic electron tunneling spectroscopy?

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Help in designing LAMP primers targeting conserved regions across six different fungal species ?

Greatly appreciate any suggestions on LAMP primer designing services/companies

24 October 2021 9,877 3 View

Cytoplasmic staining of BCL-6 in spleen tissue of treated groups rather than nuclear?

I have 3 groups of animal apart from PBS controls where i treat them under different immunosuppressive conditions. when the animals are subjected to partial or no immunosuppressive conditions, i...

31 August 2020 9,193 5 View

In IHC - Alkaline phosphatase method, do we dry the slides or not before mounting in aqueous medium?

In IHC for double staining i use DAB and AP. DAB works fine but AP after mounting is a headache as i see precipitates and also sometimes dissolves.I have tried drying the slides a bit and then...

26 July 2020 425 2 View

How do i use a rabbit monoclonal antibody on rabbit tissues with minimal or no background staining?

I am working with rabbit spleen and as it is difficult to obtain antibodies against rabbits as most of them are raised in rabbits. I had to purchase a few antibodies raised in rabbits. But as...

17 June 2020 4,101 8 View

Background fluorescent staining through different?

I am trying stain for two membrane proteins, one cellular and one viral. But lately, i am doubting a little that the double positive staining could be because of background staining of the other...

19 November 2019 9,762 3 View

I am facing difficulty in immunofluorescence due to autofluorescence?

I am trying out immunofluorescence on rabbit spleen but the results are not fruitful due to auto-fluorescence. I tried blocking with 5%BSA for an hour still persists. I also tried sudan black but...

20 March 2019 1,767 8 View

Anybody please explain the error script in origin 8.5 As "Vars node not found"?

Script window appears while curve fitting in origin with c++. This happens when running origin 8.5 in an older version of windows (xp) and due to some reason OS cannot be changed.

24 June 2018 997 0 View

Do I always need a reference electrode for CV?

I am building a microbial fuel cell. Can I do CV for the full cell without a reference electrode? Do I always need a reference electrode for CV? Or can CV be done using the anode as working...

04 July 2016 5,714 5 View

Kindly advice me about Sr isotope analytical facility available in India or abroad. I wish to get contact details of the personnel as well.

Strontium isotope

27 April 2016 5,931 2 View

Can any experts give Pt/c potenital window in alkaline medium?

can any experts give Pt/c potenital window in alkaline medium ?

03 March 2016 2,664 2 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

1. If I can quantize the atom using this hyperbolic spiral and classical physics, could nature do the same?

If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...

07 August 2024 5,343 2 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View

Why reactivity isn't increased with more empty spots in valence shell?

If from a geometric perspective the non-halogens, non-noble gases have more empty spots in their valence shell, and the filling/exiting of any of the empty spots in the shell constitutes a...

03 August 2024 4,787 2 View

Why is the molecule's orientation with an electric field affect polarizability?

Why is the molecule's orientation with an electric field affect polarizability? Electrons are diffuse enough to be independent with respect to orientation and effect of electric field on...

03 August 2024 7,843 1 View

Why doesn't chromium 2+ ion use all its d-orbitals to receive lone pairs from six waters in [Cr(H20)6)]3+?

I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...

03 August 2024 1,370 1 View

Is it necessary to covary exogenous constructs in a structural model?

I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...

03 August 2024 6,028 4 View

Why aren't most metal oxides insoluble in water?

Why BaO (s) + H2O(l) ---> Ba 2+(aq) + 2 OH - (aq) if Oxygen loves owning all the electrons, why would it bind to hydrogen which competes with Oxygen for electron ownership more so than...

03 August 2024 5,392 1 View