Cytoplasmic staining of BCL-6 in spleen tissue of treated groups rather than nuclear?

I have 3 groups of animal apart from PBS controls where i treat them under different immunosuppressive conditions. when the animals are subjected to partial or no immunosuppressive conditions, i...

31 August 2020 9,012 5 View

In IHC - Alkaline phosphatase method, do we dry the slides or not before mounting in aqueous medium?

In IHC for double staining i use DAB and AP. DAB works fine but AP after mounting is a headache as i see precipitates and also sometimes dissolves.I have tried drying the slides a bit and then...

26 July 2020 219 2 View

How do i use a rabbit monoclonal antibody on rabbit tissues with minimal or no background staining?

I am working with rabbit spleen and as it is difficult to obtain antibodies against rabbits as most of them are raised in rabbits. I had to purchase a few antibodies raised in rabbits. But as...

17 June 2020 3,930 8 View

Background fluorescent staining through different?

I am trying stain for two membrane proteins, one cellular and one viral. But lately, i am doubting a little that the double positive staining could be because of background staining of the other...

19 November 2019 9,633 3 View

I am facing difficulty in immunofluorescence due to autofluorescence?

I am trying out immunofluorescence on rabbit spleen but the results are not fruitful due to auto-fluorescence. I tried blocking with 5%BSA for an hour still persists. I also tried sudan black but...

20 March 2019 1,599 8 View

Do I always need a reference electrode for CV?

I am building a microbial fuel cell. Can I do CV for the full cell without a reference electrode? Do I always need a reference electrode for CV? Or can CV be done using the anode as working...

04 July 2016 5,531 5 View

Can any experts give Pt/c potenital window in alkaline medium?

can any experts give Pt/c potenital window in alkaline medium ?

03 March 2016 2,514 2 View

Can I still consider and amplicon peak with very low fluorescence intensity?

I am standardizing pcr and melt conditions for a particular exon. The fluorescence intensity of my amplificon is low, when compared to my other exons (picture below). Can I consider such melt...

25 November 2014 4,632 3 View

How to test Tensile on single jute fibre and what is standard size of specimen?

01 January 1970 6,081 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

Magnetismic Gravity in the universe?

MAGNETISMIC GRAVITY What is magnetism? Something magnetic has a + and - charge. If 2 + or - are pushed if + and - are pulled. But what is the main reason for this? Why does magnetism have a pull...

21 February 2021 9,538 3 View

How can I simulate the optical absorption spectrum of a biomaterial (like DNA or protein) in COMSOL?

I need to simulate the optical properties of a biomaterial, namely DNA, in COMSOL. Basically I need to simulate the IR absorption spectrum of a cylinder filled with a 'concentration' of DNA. I am...

21 February 2021 3,043 3 View