Cytoplasmic staining of BCL-6 in spleen tissue of treated groups rather than nuclear?

I have 3 groups of animal apart from PBS controls where i treat them under different immunosuppressive conditions. when the animals are subjected to partial or no immunosuppressive conditions, i...

31 August 2020 9,012 5 View

In IHC - Alkaline phosphatase method, do we dry the slides or not before mounting in aqueous medium?

In IHC for double staining i use DAB and AP. DAB works fine but AP after mounting is a headache as i see precipitates and also sometimes dissolves.I have tried drying the slides a bit and then...

26 July 2020 219 2 View

How do i use a rabbit monoclonal antibody on rabbit tissues with minimal or no background staining?

I am working with rabbit spleen and as it is difficult to obtain antibodies against rabbits as most of them are raised in rabbits. I had to purchase a few antibodies raised in rabbits. But as...

17 June 2020 3,930 8 View

Background fluorescent staining through different?

I am trying stain for two membrane proteins, one cellular and one viral. But lately, i am doubting a little that the double positive staining could be because of background staining of the other...

19 November 2019 9,633 3 View

I am facing difficulty in immunofluorescence due to autofluorescence?

I am trying out immunofluorescence on rabbit spleen but the results are not fruitful due to auto-fluorescence. I tried blocking with 5%BSA for an hour still persists. I also tried sudan black but...

20 March 2019 1,599 8 View

Do I always need a reference electrode for CV?

I am building a microbial fuel cell. Can I do CV for the full cell without a reference electrode? Do I always need a reference electrode for CV? Or can CV be done using the anode as working...

04 July 2016 5,531 5 View

Can any experts give Pt/c potenital window in alkaline medium?

can any experts give Pt/c potenital window in alkaline medium ?

03 March 2016 2,514 2 View

Can I still consider and amplicon peak with very low fluorescence intensity?

I am standardizing pcr and melt conditions for a particular exon. The fluorescence intensity of my amplificon is low, when compared to my other exons (picture below). Can I consider such melt...

25 November 2014 4,632 3 View

How to test Tensile on single jute fibre and what is standard size of specimen?

01 January 1970 6,081 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Can I download BibExcel for MacOs?

Hi everyone, I would like to ask how I can download BibExcel software for MacOs? There are some links which are supported by Windows but I could not find it for Mac. or is there any other software...

01 March 2021 652 1 View

How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Anyone with some Transgenomic Wavemaker software? Seeking help from the chromatography community?

I am trying to seek out some Transgenomic Wavemaker software (happy to purchase a computer too) to operate a fraction collector. The only way to connect to the fraction collector so far is to...

23 February 2021 9,181 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Bayesian decision trees and bayesian regression trees in Python and/or R?

I am looking for implementation of bayesian decision trees and bayesian regression trees in Python and/or R.. Do you know if the existing libraries support this

23 February 2021 6,689 3 View