I'm working on green synthesis of silver nanoparticles. To isolate Ag nanoparticles, I have tried to centrifuge the solution (after confirming the synthesis) at different speeds and for different periods of time, but so far no success.
Are you using a microcentrifuge? If so try adding some NaCl solution to decrease the zeta potential. Have you measured size and zeta potential?
This will depend very heavily on what the organic capping agent is. In many cases, Ag NPs with charged capping ligands can be precipitated by shifting the pH of the solution toward their isoelectric point.
You are not getting nanoparticles on centrifugation Abdounasser Albasher Omar ?
No Muhammad Ismail. I get some matter in the bottom of the tube thats hard to isolate; like a colloidal solution
store the solution in room temperature for 24 hours for the complete settlement of nanoparticles. After 24 hours centrifuge the reaction mixture, discard the supernatant. Add 1ml of distilled water to the pellet and resolve it ultrasonicly, then centrifuge. repeat the last step using ethanol. discard the supernatant and let the pallet dry at room temperature.
In case of other nanoparticles except Silver NPs, we even get the pellet at 4000 rpm. But in case of silver we can only get efficient pellet at 10000 or even 14000 rpm. Increase the time (25 mints) and rpm (10000-14000 rpm.). Carefully wash the pellet with ethanol. Than you will get results.
Good luck to you.
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