The bases of Gel Electrophoresis must be understood
The statement is perfectly true. SDS page is used for the separation of DNA in smaller fragments as well as proteins.
08 September 2016 7,750 1 View
Market-Based instruments in internalising externalities must be understood.
08 September 2016 8,846 2 View
Species diversity as level of diversity must be understood.
07 August 2016 5,145 6 View
The principle and application of light and electron microscope must be understood.
07 August 2016 5,656 0 View
A better understanding of phyto-remediation.
07 August 2016 2,344 5 View
The resolution of light microscope must be understood.
07 August 2016 3,089 4 View
The fundamentals Gel electrophoresis must be understood.
07 August 2016 2,148 5 View
The principle and application of light and electron microscope must be understood
07 August 2016 10,062 2 View
The bases of HTC must be understood.
07 August 2016 1,870 2 View
Bases of light and electronic microscopy must be understood.
07 August 2016 9,813 1 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...
05 August 2024 9,805 3 View
We assume this to be true. We also assume that the vacuum bomb is the latest version of explosives with an explosive power of a few to ten kg of TNT equivalent. It has the unique characteristic of...
04 August 2024 4,534 1 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View