In Gel Electrophoresis SDS-PAGE is meant for the separation of DNA in smaller fragments and proteins. Is it true?

08 September 2016 10,035 1 View

Besides environmental taxes , bargaining solution and tradable permits are market-based instruments in internalising externalities. Is it true?

08 September 2016 8,742 2 View

Light microscope uses a glass lense whereas electron microscope uses an electromagnetic lense. Is this statement true?

07 August 2016 5,548 0 View

Shannon-Wiener and Simpson Index are instrumental in the analysis of species evenness and equity in distribution. Is this statement true?

07 August 2016 5,003 6 View

A light microscope uses a glass slide whereas an electron microscope uses an electromagnetic slide . Is it true?

07 August 2016 9,943 2 View

The best light microscope has a resolution of 0.2 micro meters which is equivalent to 200 nanometers. Is this statement true?

07 August 2016 2,939 4 View

How distinguishing are the uses of agarose gel and SDS page in Gel electrophoresis?

07 August 2016 2,038 5 View

A process in which heavy metals in the soil are immobilised by plant roots is known as phyto-stablization. Is the statement true?

07 August 2016 2,223 5 View

Is it realistic to say that High Performance Liquid Chromatography is instrumental in forensic analysis of drugs and explosives?

07 August 2016 1,768 2 View

High Performance Liquid Chromatography and Paper Chromatography work based on the same principle. Is it true.?

07 August 2016 3,516 2 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View